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. 2019 Jan 29;9:858. doi: 10.1038/s41598-018-37225-y

Figure 3.

Figure 3

X-NANA, UEA-1 or MHC class II, and AIRE staining of isolated thymic stromal cells. These cells were prepared from three thymuses (C57BL/6 male, 6 W) by enzyme digestion, and the epithelial rich fraction (E2) was used. Image groups (A–E) and (F–J) are stained with X-NANA, FITC-UEA-1, and anti-AIRE coupled with Rhodamine RedTM-X secondary antibody but shown different fields. Image group (K–O) was stained with FITC-anti-MHC class II instead of FITC-UEA-1. (A,F and K), X-NANA (blue); (B,G and L), UEA-1 or MHC class II (green); (C,H and M), AIRE (red). (D,I and N) are the merged images of the three colors; (E,J and O) are DIC images. Scale bars in A, F and K are 5 μm. Cell 1 was X-NANA, UEA-1 and AIRE positive cell, cell 2 was X-NANA negative, UEA-1 and AIRE positive cell, cell 3 was X-NANA and AIRE positive, but UEA-1 negative cell, cell 4 was X-NANA and AIRE negative but UEA-1 positive, and cell 5 was X-NANA, MHC class II and AIRE positive. Cell (1) expressed weaker X-NANA sialidase activity than that of cell 1.