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. 2019 Jan 29;9:858. doi: 10.1038/s41598-018-37225-y

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© The Author(s) 2019

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Relative expression levels of Aire and the four sialidases in fractionated cells from C57BL/6. (A) relative expression levels of Neu1 and Neu3 in total thymocytes (T, white bar), T-single cell (S, diagonal lined bar), T-aggregated cell (A, vertical lined bar), residual non-T (R, black bar), first enzyme treated (E1, grid bar), and second and third enzyme treated fractions (E2, checked pattern bar) from C57BL/6 mice (male, 6 W) compared to the expression levels of β-actin. (B–D) relative expression levels of Neu4, Neu2, and Aire in the six cell fractions. Total brain RNA was used as the positive control in the reaction of Neu4 (dotted bar). All values are the averages of duplicates.