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. 2019 Jan 29;9:838. doi: 10.1038/s41598-018-37107-3

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© The Author(s) 2019

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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DH31 expression in tim-Gal4-expressing neurons or PDF expression in LNvs rescued severe arrhythmicity in Dh31-Pdf double mutants. (A-C) Comparison of free-running rhythms in different genotypes: w¹¹¹⁸, Dh31^#51, Pdf⁰¹ and Dh31^#51;Pdf⁰¹ (A), UAS-Dh31/+, tim-Gal4/+ and tim-Gal4 > UAS-Dh31 from the Dh31^#51;Pdf⁰¹ double-mutant background (B) and UAS-Pdf /+, Pdf-Gal4/+ and Pdf-Gal4 > UAS-Pdf from the Dh31^#51;Pdf⁰¹ double-mutant background (C). The data in Fig. 2A are the reproduced from Fig. 1A. The proportions of rhythmic (gray bar) and arrhythmic (white bar) flies over 10 days in DD were compared via χ² analysis. ****P < 0.0001. Numbers in the bar graphs represent the number of flies.