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. 2019 Jan 29;9:838. doi: 10.1038/s41598-018-37107-3

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© The Author(s) 2019

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Dh31 mutation did not enhance the abnormal molecular oscillations caused by Pdf mutation. The average levels of VRI expression in 10 brain hemispheres in each subgroup of clock cells (LNv, LNd and DN1) among WT, Dh31^#51, Pdf⁰¹ and Dh31^#51;Pdf⁰¹ double mutants at the indicated time points ((circadian time (CT) 1, 7, 13 and 19) in DD3. LNv (A), LNd (B) and DN1 (C). The detailed data of the average intensity are shown in Table S5 The variation of the average intensity in a day in each genotype was compared using two-way ANOVA and Sidak’s multiple-comparison test. The results for comparisons with WT flies at each time point are shown: ****P < 0.0001, ***P < 0.001, **P < 0.01 or *P < 0.05. The remaining comparisons are shown in Table S4.