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. 2019 Jan 29;9:872. doi: 10.1038/s41598-018-37318-8

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© The Author(s) 2019

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Elimination of Mincle mRNA by depletion of neutrophils and macrophages/monocytes from injured SN cells. (a) Representative flow cytometry dot plots of Ly6G⁺ and/or F4/80⁺ cells and (b) the proportions of these cells in samples of contralateral total, ipsilateral total, ipsilateral depleted fraction, and ipsilateral trapped fraction cells 12 h after PNI. (a) Lower-right (red frame) and upper-left (blue frame) quadrant of the panels representing neutrophils (Ly6G⁺) and macrophages/monocytes (F4/80⁺), respectively. Note that neutrophils and macrophages/monocytes were adequately removed from the ipsilateral depleted fraction (lower-left panel in a and lower panels in b). (c) The Mincle mRNA expression in each sample. The expression level of Mincle gene was normalized to that of Gapdh gene. PCR amplifications were done in triplicate. Data are representative of two independent experiments. Note that Mincle mRNA expression in the ipsilateral depleted fraction was virtually eliminated.