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. 2019 Jan 29;9:872. doi: 10.1038/s41598-018-37318-8

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© The Author(s) 2019

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Normal leucocyte infiltration at the injured site of Mincle^−/− mice. Representative flow cytometry dot plot of CD11b⁺Ly6G⁺ cells in the SN (a) and DRG (b) 1 day after PNI. Upper-right quadrant (red frame) of the left panels representing infiltrating neutrophils (CD11b⁺Ly6G⁺). The bar graph (right) summarises the proportions of neutrophils in the ipsilateral or contralateral tissues of WT or Mincle^−/− mice (n = 5 in each group, one-way ANOVA followed by Tukey’s post hoc test, ***P < 0.001 vs. contralateral side). Representative flow cytometry dot plot of CD11b⁺F4/80⁺ cells in the SN (c) and DRG (d) 3 days after PNI. Upper-right quadrant (red frame) of the left panels representing infiltrating monocytes/macrophages (CD11b⁺F4/80⁺). The bar graph (right) summarises the proportions of monocytes/macrophages in the ipsilateral or contralateral tissues of WT or Mincle^−/− mice (n = 4 in each group, one-way ANOVA followed by Tukey’s post hoc test, **P < 0.01 vs. contralateral side). Values are mean ± SEM.