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. 2019 Jan 29;9:872. doi: 10.1038/s41598-018-37318-8

Figure 7.

Figure 7

Mincle deficiency inhibits complete activation of spinal microglia after PNI. (a) Representative images showing Iba1-immunoreactivity in the L4 spinal dorsal horn of WT and Mincle−/− mice 7 days after PNI (Scale bar: 100 μm). (b) Number of Iba1-immunoreactive cells counted in the ipsilateral and contralateral dorsal horn of WT and Mincle−/− mice (one-way ANOVA followed by Tukey’s post hoc test, ##P < 0.01 vs. contralateral side). Real-time PCR analysis of mRNA of proinflammatory cytokines (c) and neuropathic pain-related gene (d) in the ipsilateral spinal dorsal horn of WT and Mincle−/− mice before (naïve) and after PNI. Bar graphs show fold-change compared to naïve WT mice (n = 3–7 in each group, one-way ANOVA followed by Tukey’s post hoc test. **P < 0.01 vs. WT mice, #P < 0.05, ##P < 0.01 vs. naïve controls). Values are mean ± SEM.