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. 2019 Jan 29;9:853. doi: 10.1038/s41598-018-36600-z

Figure 2.

Figure 2

Decrease of total glutathione in hypoxia. (A) Relative quantification of glutathione (GSH)-related compounds in the cells in aerobic condition and hypoxia. The detected GSH compounds are reduced GSH, its oxidized form synthesized by covalent disulfide bond between two GSH molecules (GSSG), and their persulfidated derivatives GS-SH, GS-S2H, GS-S-SG, and GS-S2-SG. Procedure defined unit is calculated by normalizing the LC/MS signals to that of aerobic conditions for each compound. Error bars indicate SD. n = 3, *p < 0.05 t-test, **p < 0.05 t-test with Bonferroni’s correction. (B) An illustration of the reduction and oxidization network of glutathione. Red and blue arrows indicate the alteration in redox state of the compounds. The superscript for each compound indicates its upregulation (UP) or downregulation (DOWN) with or without a mark indicating the significant difference (*p < 0.05 t-test, **p < 0.05 t-test with Bonferroni’s correction).