Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2019 Jan 29;9:920. doi: 10.1038/s41598-018-37000-z

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

© The Author(s) 2019

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

PMC Copyright notice

High ROS, DNA damage and the impairment of DNA damage repair in ATII cells in emphysema patients. ROS levels were analyzed in freshly isolated ATII cells from control non-smokers (NS), smokers (SM) and emphysema patients (EM) using DCF staining by flow cytometry. Representative images of flow cytometry profiles (A) and quantification (B) are shown. Representative Western blot images of OGG1 expression (C) and densitometric quantification of OGG1 expression normalized to GAPDH and non-smokers (D). Comet assay was used to detect DNA damage in freshly isolated ATII cells (E). Quantification of the Olive Tail Moment is also shown (F). Data are shown as mean values ± s.e.m. (N = 6 per group; *p < 0.05; ***p < 0.001, magnification 10 × 40).