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. 2019 Jan 29;9:920. doi: 10.1038/s41598-018-37000-z

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© The Author(s) 2019

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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DJ-1 and XLF interaction in freshly isolated ATII cells from patients with emphysema identified by mass spectrometry analysis. DJ-1 was co-immunoprecipitated in ATII cells obtained from non-smokers (NS), smokers (SM) and patients with emphysema (EM) followed by mass spectrometry analysis. The presence of DJ-1 (A) and its interaction with XLF (B) is shown in emphysema (N = 3 per group). Results were validated by co-immunoprecipitation of DJ-1 followed by Western blotting analysis of XLF expression in ATII cells obtained from non-smokers, smokers and patients with emphysema (C) and densitometric quantification (N = 6 per group; *P < 0.05) (D). Data is shown as means ± s.e.m.