Figure 6.
XLF and DJ-1 expression in lung tissue obtained from mice exposed to cigarette smoke in vivo. Lung tissue was obtained from wild-type (WT) and DJ-1 KO mice exposed to 150 mg/m3 TSP as described in Material and Method section (CTL – control; CS-cigarette smoke). The absence of DJ-1 expression in DJ-1 KO mice was confirmed by Western blotting (A). Representative Western blot images of expression and densitometric analysis of XLF (B,C), γH2AX (D,E), p21 (F,G), 4-HNE (H,I) and caspase 3 (J,K) are shown. Densitometric quantification of protein expression was normalized to β-actin (N = 3 per group). *P < 0.05; **P < 0.001. Data is shown as means ± s.e.m.