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. 2019 Jan 29;9:863. doi: 10.1038/s41598-018-37165-7

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© The Author(s) 2019

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Representative histograms showing FL1 fluorescence intensity of cells population of L. japonicus leaves under low temperature. (A,B) Histograms of MG-1 cells under control and low temperature treatment, respectively. Blue histogram: incubation with DCF-DA without light. Light blue histogram: incubation with DCF-DA and light. (C,D) Histograms of MG-20 cells under control and low temperature treatment, respectively. Red histogram: incubation with DCF-DA without light. Orange histogram: incubation with DCF-DA and light. Grey histograms: incubation without DCF-DA or light. A total of 40.000 events were analyzed in each case.