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. 2019 Jan 29;9:863. doi: 10.1038/s41598-018-37165-7

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© The Author(s) 2019

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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ROS production quantification by flow cytometry. The DFC-DA fluorescence intensity (FL1) was calculated in each case using the formula in Materials and Methods. Values are the mean ± SD of 5 biological replicates and are expressed as arbitrary units (AUs), for each ecotype and treatment. The asterisk indicates differences between control and low temperature treatments (Student’s test, p < 0.05), for each ecotype respectively. C, Control; L, Low temperature.