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. 2019 Jan 29;9:862. doi: 10.1038/s41598-018-36925-9

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© The Author(s) 2019

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Phosphorylation of AKT/p70S6K in A549 cells treated with DK-1014. A549 cells were pre-treated with DK-1014 or DEX for 1 h and then incubated for 30 min with PMA (5 nM). (a) Phosphorylation of AKT/mTOR protein evaluated using western blot analysis and (b) quantified using ImageJ. (c) Immunocytochemical staining with anti-phosphoAKT antibodies for A549 cells that were DK-1014 treated or inhibitors (magnification = ×100, bar; 100 μm). ^#p < 0.05 compared to DMSO-treated cells; **p < 0.005 compared to PMA-treated cells.