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. 2019 Jan 29;9:896. doi: 10.1038/s41598-018-36751-z

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© The Author(s) 2019

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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The feasibility of SEA to detect M. pneumoniae. (A) The real-time fluorescence curves. (B) Native PAGE of the corresponding amplification products (Supplementary Fig. S3. Lane 1, lane 2 and lane 6) (C) Colorimetric SEA assay using a pH sensitive dye as the indicator. 1 represented the vector plasmid DNA containing 16S rDNA sequence of M. pneumoniae M129, 2 represented genomic DNA extracted from sputum specimen infected by M. pneumoniae, 3 represented no template control, and M represented the 20-bp ladder marker (TaKaRa).