Figure 2.

Methylation status of LAMA2 in the normal pituitary and nonaggressive and aggressive pituitary adenomas.

(a) Description of the methylated region of the LAMA2 promoter; (b) a representative methylation pattern of the CpGs in tumor tissues with different levels of invasiveness after bisulfite treatment; each line represents one PCR product, and the PCR products are shown for each sample. ●: methylated CpG; ○: unmethylated CpG; (c) percent methylation of the LAMA2 promoter region.

All data are presented as means ± SEM; ^***p < 0.001.

CpG, cytosine–phosphate–guanosine islands; GC, ; LAMA2, laminin subunit alpha 2; PCR, polymerase chain reaction; SEM, standard error of the mean.