Figure 4.

Increased LAMA2 expression suppresses the invasion of GH3 cels.

(a) Western blotting showing the LAMA2 levels in the control group, Lenti-LAMA2 group and 5-aza-2-deoxycytidine treatment group; (b) According to the results of the transwell assay, the number of invaded cells in the LAMA2 overexpression group was less than 40% of the number in the control group; (c, d) increased protein levels of epithelial markers (E-cadherin) was observed using western blotting (C), and decreased expression of genes related to migration and invasion (N-cadherin, β-catenin, MMP-9, and Snail) was observed using RT-qPCR (d) in cells overexpressing LAMA2 or treated with 5-aza-2-deoxycytidine; GAPDH was used as a positive control.

n = 3; ^*p < 0.05 and ^**p < 0.01.

GH3, ; GAPDH, ; LAMA2, laminin subunit alpha 2; MMP-9, metalloproteinase-9; RT-qPCR, quantitative real-time polymerase chain reaction.