TABLE 1.
Differences in relative abundances of bacterial families present in ileal and cecal digesta of low- and high-RFI broiler chickens fed either ad libitum or restrictivelya
| Family | % Relative abundance |
SEM |
P value |
|||||
|---|---|---|---|---|---|---|---|---|
|
Ad libitum feeding |
Restrictive feeding |
|||||||
| Low RFI | High RFI | Low RFI | High RFI | FL | RFI | FL × RFI | ||
| Ileum | ||||||||
| o__Clostridiales;f__ | 3.58 | 0.83 | 6.83 | 11.27 | 4.229 | 0.112 | 0.843 | 0.400 |
| f__Turicibacteraceae | 42.20 | 48.27 | 29.06 | 27.85 | 8.661 | 0.058 | 0.780 | 0.676 |
| f__Ruminococcaceae | 0.95 | 0.22 | 4.49 | 2.47 | 1.642 | 0.084 | 0.408 | 0.695 |
| f__Enterobacteriaceae | 37.49 | 22.83 | 17.74 | 11.14 | 7.155 | 0.033 | 0.143 | 0.576 |
| f__Lactobacillaceae | 9.38 | 22.13 | 35.34 | 33.30 | 9.064 | 0.046 | 0.557 | 0.419 |
| f__Lachnospiraceae | 0.22 | 0.063 | 0.76 | 0.49 | 0.284 | 0.096 | 0.453 | 0.832 |
| f__Peptostreptococcaceae | 3.65 | 3.50 | 2.56 | 6.15 | 1.699 | 0.647 | 0.316 | 0.277 |
| f__Clostridiaceae | 2.15 | 1.83 | 2.60 | 6.62 | 2.247 | 0.249 | 0.414 | 0.339 |
| o__RF39;f__ | 0.023 | 0.008 | 0.060 | 0.071 | 0.033 | 0.134 | 0.949 | 0.700 |
| f__Christensenellaceae | 0.050 | 0.0012 | 0.091 | 0.036 | 0.039 | 0.330 | 0.185 | 0.934 |
| f__Bacillaceae | 0.062 | 0.018 | 0.090 | 0.044 | 0.047 | 0.560 | 0.337 | 0.989 |
| o__Streptophyta;f__ | 0.13 | 0.10 | 0.23 | 0.18 | 0.086 | 0.289 | 0.665 | 0.912 |
| o__Clostridiales; Other | 0.025 | 0.028 | 0.019 | 0.033 | 0.009 | 0.942 | 0.342 | 0.559 |
| f__Dehalobacteriaceae | 0.0004 | 0 | 0.016 | 0.005 | 0.005 | 0.054 | 0.292 | 0.336 |
| f__Erysipelotrichaceae | 0.005 | 0.006 | 0.007 | 0.014 | 0.004 | 0.281 | 0.419 | 0.436 |
| f__Coriobacteriaceae | 0.113 | 0.100 | 0.090 | 0.060 | 0.019 | 0.103 | 0.258 | 0.673 |
| f__Enterococcaceae | 0.037 | 0.031 | 0.027 | 0.071 | 0.0181 | 0.394 | 0.298 | 0.172 |
| f__Corynebacteriaceae | 0.0006 | 0.007 | 0.003 | 0.078 | 0.0351 | 0.296 | 0.252 | 0.329 |
| Ceca | ||||||||
| o__Clostridiales;f__ | 51.67 | 51.87 | 65.29 | 66.09 | 3.217 | <0.001 | 0.876 | 0.926 |
| f__Turicibacteraceae | 0.35 | 0.97 | 0.12 | 0.016 | 0.228 | 0.012 | 0.263 | 0.119 |
| f__Ruminococcaceae | 29.00 | 29.54 | 23.37 | 25.23 | 2.358 | 0.040 | 0.614 | 0.781 |
| f__Enterobacteriaceae | 7.82 | 6.62 | 2.34 | 0.90 | 1.571 | <0.001 | 0.406 | 0.940 |
| f__Lactobacillaceae | 0.96 | 0.73 | 1.17 | 1.11 | 0.326 | 0.373 | 0.662 | 0.798 |
| f__Lachnospiraceae | 7.94 | 7.65 | 5.24 | 4.16 | 0.978 | 0.003 | 0.487 | 0.688 |
| f__Peptostreptococcaceae | 0.062 | 0.048 | 0.022 | 0.010 | 0.011 | <0.001 | 0.160 | 0.906 |
| f__Clostridiaceae | 0.18 | 0.41 | 0.04 | 0.06 | 0.129 | 0.059 | 0.348 | 0.400 |
| o__RF39;f__ | 0.61 | 0.90 | 1.00 | 0.69 | 0.190 | 0.648 | 0.975 | 0.117 |
| f__Christensenellaceae | 0.52 | 0.17 | 0.65 | 0.49 | 0.123 | 0.079 | 0.047 | 0.427 |
| f__Bacillaceae | 0.38 | 0.42 | 0.19 | 0.76 | 0.179 | 0.661 | 0.095 | 0.142 |
| o__Streptophyta;f__ | 0 | 0 | 0 | 0.0008 | 0.0004 | 0.339 | 0.308 | 0.339 |
| o__Clostridiales; Other | 0.072 | 0.106 | 0.104 | 0.078 | 0.031 | 0.938 | 0.887 | 0.343 |
| f__Dehalobacteriaceae | 0.084 | 0.079 | 0.135 | 0.136 | 0.019 | 0.007 | 0.938 | 0.863 |
| f__Erysipelotrichaceae | 0.086 | 0.165 | 0.072 | 0.073 | 0.033 | 0.113 | 0.226 | 0.243 |
| f__Coriobacteriaceae | 0.005 | 0.006 | 0.007 | 0.014 | 0.004 | 0.281 | 0.419 | 0.436 |
| f__Enterococcaceae | 0.005 | 0.005 | 0.002 | 0.003 | 0.003 | 0.069 | 0.753 | 0.615 |
| f__Corynebacteriaceae | 0 | 0.000003 | 0 | 0.0006 | 0.0002 | 0.150 | 0.145 | 0.150 |
Data are presented as least-squares means and pooled standard errors of the means (SEM). n = 7 per FL group, residual-feed-intake (RFI) rank, and sex except for n = 8 high-RFI ad libitum females. RFI was calculated for the experimental period from 9 to 30 days posthatch. FL, feed intake level.