TABLE 1.
Plasmids and strains used in this study
| Plasmid or strain | Parent strain | Descriptiona | Reference or source |
|---|---|---|---|
| Plasmids | |||
| pTRKL2 | E. coli-Streptococcus shuttle vector | 30 | |
| pCN143 | Temperature-sensitive vector | 30 | |
| pMW506 | pSF152::covRΔcm | 30 | |
| pCN114 | pSF152::covSΔcm | This study | |
| pCN129 | pCN143::CovRD53A | 30 | |
| pCN160 | pCN143::covSΔcm | This study | |
| pCN167 | pCN143::CovRT65A (covR with A203G substitution) | This study | |
| pCN169 | pTRKL2::CovRD53A | This study | |
| pCN176 | pCN143::CovRD53A-T65A | This study | |
| pCN180 | pTRKL2::CovRT65A | This study | |
| Strains | |||
| A20 | emm1-type wild-type strain | 40 | |
| NZ131 | emm49-type wild-type strain | 40 | |
| A20 ΔcovS | A20 | A20 covS isogenic mutant | This study |
| A20 ΔcovR | A20 | A20 covR isogenic mutant | 30 |
| NZ131 ΔcovS | NZ131 | NZ131 covS isogenic mutant | This study |
| NZ131 ΔcovR | NZ131 | NZ131 covR isogenic mutant | This study |
| A20CovR(D53A) mutant | A20 | CovRD53A mutant strain | 30 |
| NZ131CovR(D53A) mutant | NZ131 | CovRD53A mutant strain | This study |
| A20CovR(T65A) mutant | A20 | CovRT65A mutant strain | This study |
| NZ131CovR(T65A) mutant | NZ131 | CovRT65A mutant strain | This study |
| A20CovR(D53A-T65A) mutant | A20 | CovRD53A and CovRT65A mutant strain | This study |
a
cm, chloramphenicol cassette.