TABLE 1.
E. coli strains and plasmids
| Plasmid or strain | Description | Source or reference |
|---|---|---|
| Plasmids | ||
| pDSW210 | Derivative of pBR322 with weakened Ptrc promoter | 49 |
| pWM2785 | pDSW210 expressing FLAG-FtsA | 10 |
| pEVOL | Cmr P15a plasmid expressing tRNA synthetase and tRNA for pBpA | 39 |
| pPK9 | pSC101 derivative, Spcr carrying araC and BAD promoter | P. Christie |
| pPK9-ZipA | pPK9 with zipA-Strep-Tag inserted between NotI and HindIII | This study |
| pUT18c | High-copy-no. bacterial two-hybrid vector, Apr | 50 |
| pKT25 | Medium-copy-no. bacterial two-hybrid vector, Kanr | 50 |
| pKNT25 | Medium-copy-no. bacterial two-hybrid vector, Kanr | 50 |
| pKNT25-ZipA | Bacterial two-hybrid plasmid expressing ZipA | 51 |
| pKNT25-FtsZ | Bacterial two-hybrid plasmid expressing FtsZ | 50 |
| pWM3014 | pKT25-FtsA | 10 |
| pWM3183 | pKT25-FtsN | 10 |
| pWM3021 | pUT18c-FtsA | 10 |
| pWM6134 | pUT18c-FtsA ΔR260 | This study |
| pWM6135 | pUT18c-FtsA ΔA256-R260 | This study |
| pWM6136 | pUT18c-FtsA ΔD253-R260 | This study |
| pWM5265 | pDSW210-ZipA-GFP | 21 |
| Strains | ||
| WM5163 | MG1655 WT E. coli K-12 strain | Lab collection |
| WM1074 | lac-deficient derivative of MG1655 (ilvG rpb-50 rph-1 ΔlacU169) | Lab collection |
| WM1115 | WM1074 ftsA12(Ts) | Lab collection |
| WM1125 | WM1074 ftsZ84(Ts) | Lab collection |
| WM1657 | ftsA-R286W ΔzipA::aph Kanr | This study |
| WM5855 | WM1115/pEVOL/pDSW210 | This study |
| WM5856 | WM1115/pEVOL/pWM2785 | This study |
| WM5886 | WM1115/pEVOL/pWM2785-R122X | This study |
| WM5857 | WM1115/pEVOL/pWM2785-E124X | This study |
| WM5858 | WM1115/pEVOL/pWM2785-R126X | This study |
| WM5887 | WM1115/pEVOL/pWM2785-R153X | This study |
| WM5888 | WM1115/pEVOL/pWM2785-R177X | This study |
| WM5889 | WM1115/pEVOL/pWM2785-R202X | This study |
| WM5890 | WM1115/pEVOL/pWM2785-R260X | This study |
| WM5891 | WM1115/pEVOL/pWM2785-R286X | This study |
| WM5892 | WM1115/pEVOL/pWM2785-R300X | This study |
| WM5998 | WM1115/pEVOL/pWM2785-P251X | This study |
| WM5999 | WM1115/pEVOL/pWM2785-S252X | This study |
| WM5964 | WM1115/pEVOL/pWM2785-D253X | This study |
| WM6000 | WM1115/pEVOL/pWM2785-A254X | This study |
| WM6001 | WM1115/pEVOL/pWM2785-E255X | This study |
| WM5965 | WM1115/pEVOL/pWM2785-A256X | This study |
| WM6002 | WM1115/pEVOL/pWM2785-I257X | This study |
| WM5908 | WM1115/pEVOL/pWM2785-K258X | This study |
| WM5909 | WM1115/pEVOL/pWM2785-V259X | This study |
| WM5910 | WM1115/pEVOL/pWM2785-H261X | This study |
| WM5911 | WM1115/pEVOL/pWM2785-G262X | This study |
| WM5949 | WM1657/pEVOL/pWM2785 | This study |
| WM5950 | WM1657/pEVOL/pWM2785-R260X | This study |
| WM5951 | WM1657/pEVOL/pWM2785/pPK9-ZipA | This study |
| WM5952 | WM1657/pEVOL/pWM2785-R260X/pPK9-ZipA | This study |
| WM5970 | WM1115/pWM2785(R286W)-R260X | This study |
| WM5971 | WM1657/pWM2785(R286W)-R260X | This study |
| WM5972 | WM1657/pWM2785(R286W)-R260X/pPK9-ZipA | This study |
| WM5991 | WM1115 ΔmrcA::kan/pWM2785-R260X (P1 JW3359 × WM5890) | This study |
| WM5992 | WM1115 ΔmrcB::kan/pWM2785-R260X (P1 JW0145 × WM5890) | This study |
| WM6101 | WM1115/pWM2785(D253R) | This study |
| WM6102 | WM1115/pWM2785(D253W) | This study |
| WM6103 | WM1115/pWM2785(A256R) | This study |
| WM6104 | WM1115/pWM2785(A256W) | This study |
| WM5935 | WM1115/pWM2785(R260A) | This study |
| WM5936 | WM1115/pWM2785(R260E) | This study |
| WM5937 | WM1115/pWM2785(R260W) | This study |
| WM6105 | WM1115/pWM2785(Δ253) | This study |
| WM6106 | WM1115/pWM2785(Δ256) | This study |
| WM6107 | WM1115/pWM2785(Δ260) | This study |
| WM6108 | WM1115/pWM2785(Δ256-260) | This study |
| WM6109 | WM1115/pWM2785(Δ253-260) | This study |
| BTH101 | F− cya-99 araD139 galE15 galK16 rpsL1 hsdR2 mcrA1 mcrB1 | 52 |
| JW3359 | ΔmrcA::kan strain | Keio collection |
| JW0145 | ΔmrcB::kan strain | Keio collection |
| WM6234 | ftsZ-55-mCer-56 sandwich fusion replacing chromosomal ftsZ | 44 |
| WM6015 | WM1125/pEVOL/pWM2785-R260X | This study |
| WM6019 | WM5163/pEVOL/pWM2785-R260X | This study |
| WM6253 | WM6234/pEVOL/pWM2785-R260X | This study |
| WM5446 | MG1655/pDSW210-ZipA-GFP | Lab collection |