Figure 5.

Effects of expression of Scn8a^R1872W in forebrain neurons. (A) Survival of conditional mice expressing cell-specific Cre constructs. Nestin-Cre is broadly expressed in neural cells; median survival 21 days. Emx1-Cre is expressed in excitatory neurons of the forebrain; median survival 46 days. Gad2-Cre is expressed in inhibitory neurons. (B) Brain transcripts encoding arginine 1872 (173 bp RT-PCR fragment) and tryptophan 1872 (273 bp RT-PCR fragment) in mice expressing various Cre recombinases.