Skip to main content
. 2019 Jan 17;142(2):344–361. doi: 10.1093/brain/awy322

Figure 5.

Figure 5

Sephin1 reduces demyelination at EAE PID 17 and modulates ISR activity. Lysates of lumber spinal cords were analysed from PID 17 and PID 30 CFA or EAE mice treated with vehicle or Sephin1 beginning from PID 7. (A and B) Immunoblot analysis of MBP and MAG, and the key ISR components CHOP, ATF4, and p-eIF2α. T- eIF2α and β-actin is presented as a loading control. Images cropped horizontally are displayed in A and images cropped horizontally and vertically displayed in B. The scanned full blots are presented in Supplementary Figs 14 and 15, respectively. (C and D) Densitometry histograms of the ISR component proteins after normalization to β-actin or T-eIF2α, respectively. (E and F) Densitometry histograms of myelin proteins after normalization to β-actin. *P < 0.05, **P < 0.01, significance based on ANOVA. Data represent an average of 3–4 mice per group (mean ± SEM).