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. 2019 Jan 23;10:4. doi: 10.3389/fendo.2019.00004

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Copyright © 2019 Xu, Chen, Xiao, Wang, DiSanto and Zhang.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Knockdown of IL-21R in BPH-1 cells with siRNA. (A) Knockdown efficiency of IL-21R in the mRNA levels in BPH-1 cells with three different siRNA sequences (siIL-21R 1, 2, and 3). Boxes, mean; bars, ± SD; con = control; NS means no significance, ^**P < 0.01 vs. control-siRNA (sicon). (B) Representative Western Blot band of IL-21R in BPH-1 cells treated with siIL-21R of the highest inhibitory efficiency (siIL-21R1) and sicon. (C) Relative densitometric quantification of IL-21R in BPH-1 cells. GAPDH expression was analyzed as a loading control, results are expressed as ratio of IL-21R in respect to GAPDH. Boxes, mean; bars, ± SD; ^**P < 0.01 vs. sicon. (D) Immunofluorescence of IL-21R in BPH-1 cells treated with siIL-21R1 and sicon. Cy3-immunofluorescence (red) indicates IL-21R expression. DAPI (blue) indicates cell nuclear staining. Merged image indicates of IL-21R and DAPI. The scale bars are 100 μm.