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. 2019 Jan 23;10:4. doi: 10.3389/fendo.2019.00004

Figure 4.

Figure 4

Effect of knockdown of IL-21R on BPH-1 cell apoptosis and cell cycle. (A) Flow Cytometry analyses for cell apoptosis in BPH-1 cells treated with siIL-21R1 and sicon. (B) Bar graph for apoptosis rate of BPH-1 cells. Boxes, mean; bars, ± SD; **P < 0.01 vs. sicon. (C) Flow Cytometry analyses for cell cycle in BPH-1 cells treated with siIL-21R1 and sicon. (D) Bar graph for the percentage of BPH-1 cells in each stage. Boxes, mean; bars, ± SD; **P < 0.01 vs. sicon. (E) Left, Representative Western Blot band of cell apoptosis associated proteins (BAX and Bcl-2) in BPH-1 cells. Right, Relative densitometric quantification of cell apoptosis associated protein (BAX and Bcl-2) in BPH-1 cells. GAPDH expression was analyzed as a loading control, results are expressed as ratio of the proteins in respect to GAPDH. Boxes, mean; bars, ± SD; **P < 0.01 vs. sicon. (F) Left, Representative Western Blot band of cell cycle associated protein (Cyclin D1, CDK4, and CDK6) in BPH-1 cells. Right, Relative densitometric quantification of cell cycle associated protein (Cyclin D1, CDK4, and CDK6) in BPH-1 cells. GAPDH expression was analyzed as a loading control, results are expressed as ratio of the proteins in respect to GAPDH. Boxes, mean; bars, ± SD; **P < 0.01 vs. sicon.