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. 2019 Jan 23;10:4. doi: 10.3389/fendo.2019.00004

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Copyright © 2019 Xu, Chen, Xiao, Wang, DiSanto and Zhang.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Effect of knockdown of IL-21R on EMT process in BPH-1 cells. (A–C) Immunofluorescence of E-Cad, N-Cad, and vimentin in BPH-1 cells treated with siIL-21R1 and sicon. Left, DAPI (blue) indicates cell nuclear staining. Middle, Cy3-immunofluorescence (red) indicates E-Cad (A), N-Cad (B), and vimentin (C) expression. Right, Merged image. The scale bars for (A–C) are 100 μm. (D) Representative Western Blot bands of EMT associated protein (E-Cad, N-Cad, and vimentin) in BPH-1 cells. (E) Relative densitometric quantification of EMT associated proteins (E-Cad, N-Cad, and vimentin) in BPH-1 cells. GAPDH expression was analyzed as a loading control, results are expressed as ratio of the proteins in respect to GAPDH. Boxes, mean; bars, ± SD; ^**P < 0.01 vs. sicon.