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. 2019 Jan 23;10:4. doi: 10.3389/fendo.2019.00004

Figure 8.

Figure 8

Effect of AcTHP-1 co-culture on EMT process in BPH-1 cells. (A–C) Immunofluorescence of E-Cad (A), N-Cad, (B) and vimentin (C) in BPH-1 cells co-cultured with or without AcTHP-1 in the absence or presence of siIL-21R1. Left, DAPI (blue) indicates cell nuclear staining. Middle, Cy3-immunofluorescence (red) indicates E-Cad (A), N-Cad (B), and vimentin (C) expression. Right, Merged image. The scale bars for (A–C) are 100 μm. (D) Representative Western Blot bands of EMT associated protein (E-Cad, N-Cad, and vimentin) in BPH-1 cells. (E) Relative densitometric quantification of EMT associated protein (E-Cad, N-Cad, and vimentin) in BPH-1 cells. GAPDH expression was analyzed as a loading control, results are expressed as ratio of the proteins in respect to GAPDH. Boxes, mean; bars, ± SD; **P < 0.01 vs. BPH-1cells without AcTHP-1 co-culture in the presence of siIL-21R1. NS means no significance, BPH-1cells without AcTHP-1 co-culture in the absence of siIL-21R1 vs. BPH-1 cells with AcTHP-1 co-culture in the presence of siIL-21R1.