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. 2018 Dec 4;18(1):e12872. doi: 10.1111/acel.12872

Figure 4.

Figure 4

O‐GlcNAcylation of c‐Fos on S56 and S57 promotes neuronal cell death in the presence of Aβ. (a, b) EGFP‐c‐Fos‐WT or EGFP‐c‐Fos‐S56A‐S57A transfected SH‐SY5Y cell lines were treated with Aβ (at doses indicated) for 24 hr. Representative images of TUNEL assay (scale bar: 250 μm) (a) and quantitative graph (b) show that EGFP‐c‐Fos‐S56A‐S57A prevented Aβ‐induced cell death (n = 6). Arrows indicate TUNEL+ EGFP+ cells. (c, d) Tag free‐c‐Fos‐WT or tag free‐c‐Fos‐S56A‐S57A transfected SH‐SY5Y cell lines were treated with Aβ (at doses indicated) for 24 hr. Quantitative graphs of MTS assay (c) and Calcein‐AM assay (d) show that tag free‐c‐Fos‐S56A‐S57A transfected groups were resistant to cell death compared to tag free‐c‐Fos‐WT transfected groups in the presence of Aβ ((c): n = 8, (d): n = 8). Data are shown as mean ± SEM. ## p < 0.01, ### p < 0.001 among c‐Fos‐WT or c‐Fos‐S56A‐S57A transfected groups (one‐way ANOVA, Bonferroni posthoc test), *p < 0.05, **p < 0.01, ***p < 0.001 between c‐Fos‐WT and c‐Fos‐S56A‐S57A groups (two‐way ANOVA, Bonferroni posthoc test). S56A‐S57A: c‐Fos‐S56A‐S57A; Veh: Vehicle; WT: c‐Fos‐WT