Figure 3.

dilp1 and dilp2 interact to regulate metabolism, physiology, and compensatory dilp expression. Female flies aged 7–10 days old, 22–44 flies per replicate sample. Figure 3a–c,e,f show significance (*p < 0.05, **p < 0.01) from post hoc pairwise comparisons in two‐way ANOVA. (a) Adult mass reduced by dilp2 mutant; dilp1 × dilp2 interaction p = 0.03 (n = 5 samples per genotype). Hemolymph sugar (b) and glycogen (c) reduced by single dilp mutants, with significant dilp1 × dilp2 interaction, respectively, p < 0.001 and p = 0.005 (n = 5 samples per genotype). (d) Survival when fasted, each cohort with n = 118–150 flies, three replicate cohorts. Log‐rank tests relative to w1118, ****p < 0.0001. dilp3 (e) and dilp5 (f) mRNA are moderately induced by mutation of dilp2 but not of dilp1, with significant dilp1 × dilp2 interaction, respectively, p = 0.02 and p = 0,02 (n = 3–6 replicate samples per genotype)