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Mechanism of action of Ribozyme. A hammerhead ribozyme has two binding arms designed to bind to the complimentary RNA targets in a Watson–Crick pairing. The ribozyme binds to its target mRNA and makes an mRNA-ribozyme complex. The catalytic motif of the ribozyme then cleaves its target RNA into pieces, thus inhibiting gene expression. After cleaving the RNA target, the ribozyme becomes free again to enter into the next cycle.
