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. 2019 Jan 18;8(1):116. doi: 10.3390/jcm8010116

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© 2019 by the authors.

Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).

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Heat-killed mycobacterium tuberculosis (HKMT) induces collagen I synthesis and NOX4–ROS expression in pleural mesothelial cells (PMC) (a) Western blotting analyses of NOX4, phosphorylated ERK, collagen, and EMT markers, (b) COL1A mRNA concentration by RT-PCR, (c) representative photomicrographs of cells subjected to 6-carboxy-2’,7’-dichloroflurescin diacetate (DCFA-DA) treatment. * p < 0.01 vs. 0 h after HKMT treatment. Data are presented as the mean ± SD of three independent experiments. NOX, NADPH oxidase; EMT, epithelial to mesenchymal transition; COL1A, Collagen 1A; ROS, reactive oxygen species.