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. 2019 Jan 18;8(1):116. doi: 10.3390/jcm8010116

Figure 2.

Figure 2

Figure 2

NOX4 mediates HKMT-induced collagen-1A synthesis and EMT. Pleural mesothelial cells were transfected with SiRNA targeting NOX4 (SiNOX4). Twenty-four hours later, the cells were treated with HKMT (10 ng/mL) for 1 h or were left untreated. (a) Western blotting analyses of NOX4, phosphorylated ERK, and collagen, and EMT markers. (b) COL1A mRNA level by RT-PCR. (c) Representative photomicrographs of cells subjected to DCFA–DA treatment. * p < 0.05. Data are expressed as mean ± SD. NOX, NADPH oxidase; EMT, epithelial to mesenchymal transition; COL1A, Collagen 1A. Data are presented as the mean ± SD of three independent experiments.