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. 2019 Jan 18;8(1):116. doi: 10.3390/jcm8010116

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© 2019 by the authors.

Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).

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Activation of NOX4 and mesothelin deposition in a BCG-induced pleurisy mouse model. NOX4 and mesothelin (markers for PMCs) were subjected to immunofluorescence staining. A1–D1: DAPI staining. A2–D2: Immunofluorescence staining of mesothelin (green color). A3–D3: Immunofluorescence staining of NOX4 (red color). A4–D4: Overlays of immunofluorescence staining and DAPI staining, yellow areas represent colocalization of mesothelin and NOX4. DAPI, 4′,6-Diamidino-2-phenylindole dihydrochloride.