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. 2019 Jan 14;8(1):90. doi: 10.3390/jcm8010090

Table 1.

Primers used for the quantification of gut specific bacteria.

Bacterial Species	Primer	Nucleotide Sequence (5’-3’)
Total bacteria	Forward	GTGSTGCAYGGYTGTCGTCA
Total bacteria	Reverse	ACGTCRTCCMCACCTTCCTC
Bifidobacterium	Forward	CGCGTCYGGTGTGAAAG
Bifidobacterium	Reverse	CCCCACATCCAGCATCCA
Akkermansia muciniphila	Forward	CAGCACGTGAAGGTGGGGAC
Akkermansia muciniphila	Reverse	CCTTGCGGTTGGCTTCAGAT
Escherichia coli	Forward	CATGCCGCGTGTATGAAGAA
Escherichia coli	Reverse	CGGGTAACGTCAATGAGCAAA
Clostridium cluster I	Forward	TACCHRAGGAGGAAGCCAC
Clostridium cluster I	Reverse	GTTCTTCCTAATCTCTACGCAT
Bacteroides-Provetella	Forward	AAGGTCCCCCACATTGG
Bacteroides-Provetella	Reverse	CCGCGGCKGCTGGCAC
Proteobacteria	Forward	TGGTGTAGGGGTAAAATCCG
Proteobacteria	Reverse	AGGTAAGGTTCTTCGYGTATC
Actinobacteria	Forward	TGTAGCGGTGGAATGCGC
Actinobacteria	Reverse	AATTAAGCCACATGCTCCGCT
Fusobacteria	Forward	AAGCGCGTCTAGGTGGTTATGT
Fusobacteria	Reverse	TGTAGTTCCGCTTACCTCTCCAG
Enterococcus	Forward	CCCTTATTGTTAGTTGCCATCATT
Enterococcus	Reverse	ACTCGTTGTACTTCCCATTGT
Firmicutes	Forward	GCGTGAGTGAAGAAGT
Firmicutes	Reverse	CTACGCTCCCTTTACAC
Bacteroidetes	Forward	CTGAACCAGCCAAGTAGCG
Bacteroidetes	Reverse	CCGCAAACTTTCACAACTGACTTA