Table 9.
Measurement techniques meant for veterinary antibiotics in food and feed samples.
| Matrix | Number of Analytes/Execution Time | Extraction Method | Measurement Method, Chromatographic Column | Reference |
|---|---|---|---|---|
| Recent Multiresidue and Multi-Class Analysis of Antibiotics in Feeds | ||||
| Rendering products | 40/Not Indicated | During extraction, fat was removed and clean up performed using an SPE PRiME HLB cartridge, eluate evaporated to dryness and reconstituted with ACN and formic acid | BEH C18 column Identification: HPLC-MS/MS/ESI+ |
[201] |
| Compound feed | 3/Not Indicated | Formic acid/H2O (80:10) | Hypersil Gold HILIC (150 × 3.0 mm, 5 µm) and C18 (2.1 × 50 mm, 3.5 µm). Identification: HPLC-MS/MS/ESI+ |
[202] |
| Pig, poultry, and cattle feed | 62/13 | ACN/H2O (90:10) acidified with CH3COOH. | C18 Vensusil XBP (50 × 2.1 mm, 3.0 μm, 100 Å). Identification: HPLC-MS/MS/ESI+ | [209] |
| Feed | 10/Not Indicated | Acidic extraction with hydrochloric acid (0.5 mol L−1 aqueous solution), and purified by SPE cartridge | Acquity UPLC HSS T3 (150 × 2.1 mm, 1.7 μm). Identification: HPLC-MS/MS/ESI± |
[213] |
| Multiresidue Analysis of Antibiotics in Foods | ||||
| Fish muscle | 41/20 | Extraction with ammonium formate and ACN/H2O (80:20) | X-SELECT C18 (150 × 2.1 mm, 3.5 μm) Identification: HPLC-MS/MS/ESI± |
[205] |
| Shrimp | 24/8 | Extraction with formic acid in water and ACN | XBridge BEH C18 (100 × 2.1 mm, 2.5 μm). Identification HPLC-MS/MS/ESI+ | [206] |
| Poultry muscle tissue and eggs | 14/14 | ACN extraction Centrifugation at 0 °C 45 min |
Poroshell 120 ECC18 (50 × 3.0 mm, 2.7 μm) Identification: HPLC-MS/MS/ESI± (quadrupole linear ion trap) |
[207] |
| Honey | 6/Not Indicated | Modified QuEChERS method Extraction was performed using ACN and MgSO4 and NaCl |
ZORBAX Eclipse XDB C-18 (150 × 4.6 mm, 5 µm). Identification: HPLC-MS/MS/ESI+ | [214] |
ACN: Acetonitrile.