Figure 1.

Experimental Design. Twenty-eight-day mice with young ovaries (YO) were treated with 4-vinylcyclohexene diepoxide (VCD) or Oil for 15 days. At 60 days of age, germ cell-depleted (GD) ovaries and germ cell-containing (GC) ovaries were removed and transplanted into 400-day mice. After a minimum of one month to recover, all mice were evaluated and assessed via health span assays starting at 460 days of age. Mice were collected when the treatment groups were 700 ± 10 days for further health span analysis and evaluation.