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. 2019 Jan 2;8(1):38. doi: 10.3390/jcm8010038

Figure 2.

Figure 2

FSS affects cell motility by altering the expression of epithelial-mesenchymal transition (EMT)-related proteins and focal adhesion kinase (FAK) in TNBC cells. (A) Wound healing assays showed that high FSS inhibited migration, while low FSS promoted it in MDA-MB-231 and MDA-MB-468 cells. Scale bar: 100 μm. (B) Cell invasion assays showed that high FSS reduced the number of invasive cells, while low FSS promoted it in MDA-MB-231 and MDA-MB-468 cells. Scale bar: 200 μm. (C) FSS altered the expression of several EMT-related proteins and FAK in MDA-MB-231 and MDA-MB-468 cells. (D) Representative immunofluorescence images of FAK (green)/vimentin (red) or FAK (green)/E-cadherin (red) after low/high FSS treatment in MDA-MB-231 and MDA-MB-468 cells. Vimentin structure and focal adhesions are indicated with white arrow heads. Scale bar: 10 μm. Data from at least three experiments were used for statistical analysis and * p < 0.05.