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. Author manuscript; available in PMC: 2019 Jul 21.
Published in final edited form as: Nat Immunol. 2019 Jan 21;20(2):129–140. doi: 10.1038/s41590-018-0288-7

Figure 1. Mouse basophils express TLRs and produce TNF and IL-6 after activation with TLR ligands.

Figure 1.

(a-c) Tlr mRNA expression relative to GAPDH in FcεRIα+CD49b+c-Kit– basophils that were sorted from bone marrow-derived cells cultured in the presence of IL-3 (a) or from bone marrow cells harvested from naïve mice (b) or from mice at 24 h after CLP (c). The qPCR data are from one representative experiment of the n = 3 independent experiments performed, each of which gave similar results. (d, e) TNF (d) and IL-6 (e) concentrations in the supernatants of CD49b+ cells (1 × 106 cells) following 6 h of TLR ligand stimulation (TLR1/2 [PAM 3CSK4, 250 ng/ml], TLR2 [HKLM, 1 × 108/ml] or TLR4 [LPS, 1 μg/ml]). Data in d and e are shown as mean + SEM of the average of duplicate specimens with circles showing values obtained from individual experiments (n = 3 for medium, n = 8 for LPS and n = 5 for PAM 3CSK4 or HKLM in d, and n = 3 for medium, PAM 3CSK4 or HKLM, and n = 4 for LPS, in e. P values by Mann-Whitney test.