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. Author manuscript; available in PMC: 2020 Jan 1.
Published in final edited form as: Mol Reprod Dev. 2018 Nov 30;86(1):4–19. doi: 10.1002/mrd.23083

Figure 2. PLCζ is mostly located in the post-acrosomal area apposed to the inner acrosomal membrane.

Figure 2.

A. Immunofluorescence studies of human sperm analyzed by confocal microscopy showing the localization of PLCζ in these cells. Control human sperm were stained with hPLCζ-antibodies (green) and Hoechst to identify nuclear DNA (blue). PLCζ has a ring-like shape at the base of the acrosome and surrounding the nucleus. B. Subcellular localization of PLCζ by EM. Gold particles targeting PLCζ antigenic sites were observed in the perinuclear matrix along the inner acrosomal membrane (white arrow heads). Gold particles targeting PLCζ antigenic sites were often observed at the base of the acrosome. Adapted from Escoffier et al, 2015.