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. 2018 Dec 1;78(2):270–277. doi: 10.1136/annrheumdis-2018-213882

Figure 4.

Figure 4

Functional validation miRNA–mRNA interactions. (A) Expression of AMIGO1, SMAD3, GHR and DCAKD in primary chondrocytes transfected with miR-143–3 p mimic or antagomir compared with control as determined by RT-qPCR. (B) Expression of WNT9A, FZD1 and GDF6 in primary chondrocytes transfected with miR-329–3 p or miR-99a-3p mimic or antagomir compared with control as determined by RT-qPCR. Data shown are the average±SE of the mean for three independent donors (*p<0.05). miRNA, microRNA; mRNA, messenger RNA; RT-qPCR, reverse transcriptase-quantitative PCR.