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. 2018 Feb 2;68(2):347–358. doi: 10.1136/gutjnl-2017-315348

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Promotion of hepatocarcinogenesis by HFD is tempered by TonEBP haplodeficiency. Animals were injected with DEN as in figure 2, followed by feeding with ND or HFD for 30 weeks (online supplementary figure S7). (A) Non-tumour regions adjacent to tumours were immunoblotted for TonEBP and Hsc70 in TonEBP^+/+ animals. (B) Representative liver images. (C) Top: tumour number, maximal tumour size and maximal tumour weight from individual animals fed with ND (n=12 for each genotype) or HFD (n=15 for each genotype). Mean+SEM, *P<0.05 compared with corresponding TonEBP^+/+. ^#P<0.05 compared with corresponding ND. Bottom: tumours larger than 3 mm in diameter from representative individual animals are shown. (D) Representative images of H&E stained liver tissues from animals fed with HFD. (E) RT-qPCR analyses as in figure 2E. (F) Correlation of TonEBP mRNA expression with mRNA expression of COX-2, TNFα and MCP-1 in non-tumour areas of hepatic tissues from TonEBP^+/+ animals fed with ND (n=10) and HFD (n=10). COX-2, cyclo-oxygenase-2; DEN, diethylnitrosamine; HFD, high-fat diet; MCP-1, monocyte chemoattractant protein 1; mRNA, messenger RNA; ND, normal diet; TNFα, tumour necrosis factor alpha; TonEBP, tonicity-responsive enhancer-binding protein.