Table 1.
The percentage distribution of the control and CAK, CPK, SAK and SPK treated HT-29 cells after 24 h. The SAK and SPK extracts caused an outspoken increase in the percentage of cells that arrested in the S-phase of the cell cycle indicating a higher mean ± SD as compared to the CAK and CPK exposed cells. CPK extracts induced its highest percentage of cells in the S-phase at a concentration of 1000 μg/mL
| Time and dosage | Kernel sample | Cell cycle phases | ||
|---|---|---|---|---|
| G1 | S | G2 | ||
| 24h100μg/mL | Control | 85.2 | 13.88 | 0.91 |
| CAK T | 90.09 | 7.99 | 1.92 | |
| CAK L | 94.8 | 3.76 | 1.44 | |
| CAK H | 90.64 | 7.81 | 1.55 | |
| CPK T | 75.68 | 24.32 | 0 | |
| CPK L | 80.17 | 18.41 | 1.42 | |
| CPK H | 82.36 | 16.12 | 1.52 | |
| SAK T | 62.08 | 37.92 | 0 | |
| SAK L | 62.13 | 38.87 | 0 | |
| SAK H | 13.6 | 86.4 | 0 | |
| SPK T | 35.53 | 64.47 | 0 | |
| SPK L | 33.2 | 66.8 | 0 | |
| SPK H | 43.75 | 56.25 | 0 | |
| 24h500μg/mL | CAK T | 88.78 | 9.83 | 1.4 |
| CAK L | 84.3 | 14.43 | 1.27 | |
| CAK H | 87.6 | 10.45 | 1.94 | |
| CPK T | 87.69 | 10.31 | 2 | |
| CPK L | 86.9 | 10.67 | 2.43 | |
| CPK H | 86.69 | 11.98 | 1.33 | |
| SAK T | 39.87 | 60.13 | 0 | |
| SAK L | 79.13 | 20.87 | 0 | |
| SAK H | 34.43 | 65.57 | 0 | |
| SPK T | 65.75 | 34.25 | 0 | |
| SPK L | 78.12 | 21.88 | 0 | |
| SPK H | 49.02 | 50.98 | 0 | |
| 24h1000μg/mL | CAK T | 78.47 | 17.38 | 4.15 |
| CAK L | 78.51 | 15.88 | 5.61 | |
| CAK H | 81.06 | 16.95 | 1.99 | |
| CPK T | 57.11 | 38.17 | 4.72 | |
| CPK L | 70.1 | 29.9 | 0 | |
| CPK H | 50.67 | 42.67 | 6.65 | |
| SAK T | 39.81 | 60.19 | 0 | |
| SAK L | 84.43 | 15.57 | 0 | |
| SAK H | 25.53 | 74.47 | 0 | |
| SPK T | 89.71 | 10.29 | 0 | |
| SPK L | 42.88 | 57.12 | 0 | |
| SPK H | 50.33 | 49.67 | 0 | |
numbers in bold indicate % values almost double and more than the Controls values for cells in the S-phase