Table 2.
The percentage distribution of the control and CAK, CPK, SAK and SPK treated HT-29 cells after 48 h. The SAK and SPK extracts caused an outspoken increase in the percentage of cells that arrested in the S-phase of the cell cycle indicating a higher mean ± SD as compared to the CAK and CPK exposed cells
| Time and dosage | Kernel sample | Cell cycle phases | ||
|---|---|---|---|---|
| G1 | S | G2 | ||
| 48h100μg/mL | Control | 8.,47 | 15.3 | 1.24 |
| CAK T | 71.05 | 28.95 | 0 | |
| CAK L | 89.91 | 10.09 | 0 | |
| CAK H | 86.82 | 12.63 | 0.55 | |
| CPK T | 87.31 | 12.02 | 0.67 | |
| CPK L | 77.04 | 20.71 | 2.25 | |
| CPK H | 69.05 | 29.12 | 1.83 | |
| SAK T | 51.56 | 45.32 | 3.12 | |
| SAK L | 84.06 | 13.12 | 2.83 | |
| SAK H | 81.57 | 16.33 | 2.1 | |
| SPK T | 51.39 | 48.61 | 0 | |
| SPK L | 47.06 | 52.94 | 0 | |
| SPK H | 23.92 | 76.08 | 0 | |
| 48h500μg/mL | CAK T | 81.41 | 17.74 | 0.85 |
| CAK L | 85.68 | 12.53 | 1.79 | |
| CAK H | 83.12 | 16.33 | 0.55 | |
| CPK T | 8725 | 11.34 | 1.4 | |
| CPK L | 87.88 | 9.56 | 2.56 | |
| CPK H | 86.21 | 10.82 | 2.97 | |
| SAK T | 77.9 | 16.71 | 5.39 | |
| SAK L | 29.26 | 70.74 | 0 | |
| SAK H | 66.34 | 33.66 | 0 | |
| SPK T | 81.08 | 15.06 | 3.86 | |
| SPK L | 71.66 | 28.34 | 0 | |
| SPK H | 58.12 | 41.88 | 0 | |
| 48h1000μg/mL | CAK T | 81.14 | 15.16 | 3.71 |
| CAK L | 86.3 | 11.78 | 1.92 | |
| CAK H | 83.58 | 13.66 | 2.75 | |
| CPK T | 87.65 | 11.41 | 0.94 | |
| CPK L | 86.93 | 12.3 | 0.77 | |
| CPK H | 86.92 | 13.08 | 0 | |
| SAK T | 54.87 | 45.13 | 0 | |
| SAK L | 62.13 | 37.87 | 0 | |
| SAK H | 58.27 | 41.73 | 0 | |
| SPK T | 22.38 | 77.62 | 0 | |
| SPK L | 62.89 | 37.11 | 0 | |
| SPK H | 53.46 | 46.54 | 0 | |
numbers in bold indicate % values almost double and more than the Controls values for cells in the S-phase