Table 3.
The percentage distribution of the control and CAK, CPK, SAK and SPK treated HT-29 cells after 72 h. Although the SAK and SPK extracts induced an outspoken increase in percentage of cells that arrested in the S-phase, for most of the extractions the effect was overcome
| Time and dosage | Kernel sample | Cell cycle phases | ||
|---|---|---|---|---|
| G1 | S | G2 | ||
| 72h100μg/mL | Control | 83.51 | 15.61 | 0.88 |
| CAK T | 81.88 | 15.92 | 2.2 | |
| CAK L | 77.11 | 21.72 | 1.17 | |
| CAK H | 80.06 | 17.43 | 2.51 | |
| CPK T | 79.07 | 19.34 | 1.59 | |
| CPK L | 80.85 | 18.43 | 0.72 | |
| CPK H | 86.88 | 11.81 | 1.31 | |
| SAK T | 83.19 | 16.04 | 0.76 | |
| SAK L | 70.39 | 29.61 | 0 | |
| SAK H | 77 | 23 | 0 | |
| SPK T | 89.78 | 10.22 | 0 | |
| SPK L | 36.56 | 63.44 | 0 | |
| SPK H | 79.39 | 20.61 | 0 | |
| 72h500μg/mL | CAK T | 81.14 | 15.16 | 3.71 |
| CAK L | 86.3 | 11.78 | 1.92 | |
| CAK H | 83.58 | 13.66 | 2.75 | |
| CPK T | 86.09 | 10.99 | 2.92 | |
| CPK L | 85.08 | 12.34 | 2.57 | |
| CPK H | 43.27 | 56.73 | 0 | |
| SAK T | 89.14 | 10.86 | 0 | |
| SAK L | 85.23 | 14.77 | 0 | |
| SAK H | 79.97 | 17.73 | 2.29 | |
| SPK T | 83.64 | 16.36 | 0 | |
| SPK L | 62.86 | 37.14 | 0 | |
| SPK H | 84.42 | 15.58 | 0 | |
| 72h1000μg/mL | CAK T | 86,43 | 11.13 | 2.45 |
| CAK L | 86.39 | 10.57 | 3.04 | |
| CAK H | 87.47 | 8.86 | 3.67 | |
| CPK T | 82.31 | 16 | 1.69 | |
| CPK L | 88.12 | 9.49 | 2.39 | |
| CPK H | 87.31 | 9.24 | 3.45 | |
| SAK T | 74.9 | 24.54 | 0.56 | |
| SAK L | 82.83 | 14.9 | 2.27 | |
| SAK H | 86.51 | 11.97 | 1.53 | |
| SPK T | 66.84 | 33.16 | 0 | |
| SPK L | 89.59 | 10.41 | 0 | |
| SPK H | 96.66 | 3.23 | 0.11 | |
numbers in bold indicate % values almost double and more than the Controls values for cells in the S-phase