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. 2019 Jan 29;7:21. doi: 10.1186/s40425-019-0503-6

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© The Author(s). 2019

Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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Fig. 3 — HMGN1/αCD4 treatment increased CCR7⁺CD80^hiCD86^hi migratory DCs in the tumor. a Flow cytometry gating of CCR7⁺ migratory DCs. b, c The frequency and number of CCR7⁺ migratory DCs in the tumor. d The expression of CD80, CD86, and I-A/I-E (also known as MHC class II) on CCR7⁺ migratory DCs. Each result is representative of three independent experiments with at least four mice per group. Data are presented as mean ± SEM. *, P < 0.05, **, P < 0.01, ***, P < 0.001 for a dunnett’s post hoc test (compared with control); p values in the figure indicate Student’s t-test comparing mH/αCD4-treated and αCD4-treated groups. Mean fluorescence intensity (MFI)