FIG. 4.

In vivo delivery and efficacy of therapeutic RNAs. (A) Delivery of PHD2-sshRNAs using LbL formulation. Uptake into the dermal layer at days 2 and 6 of a fluorescent-tagged version of SG404 (SG405, red). Nuclei are stained with DAPI (blue). (E) Epidermis, (D) dermis. (B) Quantification of fluorescence uptake. (C–E) PHD2 and miR-210 inhibition improves wound healing in diabetic murine wounds. (C) Time course of wound closure showing significantly faster closure with SG404, SG608, and combination treatments compared to the untreated control. (D) Results from (C) replotted to show time to closure. (E) Representative images of full-thickness excisional wounds treated with therapeutic RNAs and controls. Photos were taken on day 0 and every 2 days thereafter (horizontal axis) until closure was complete. The orange rings are silicone splints to prevent wound contraction. *p < 0.05; **p < 0.01; ***p < 0.001. LbL, layer-by-layer.