Figure 1.

Cryo-EM Structure of the Mammalian Mitochondrial 28S-IF3_mt Complex

(A) Three-dimensional cryo-EM map of the 28S mitoribosome-IF3_mt complex, as viewed from the subunit's side that would face the large mitoribosomal subunit. The 28S subunit is shown in yellow, and the density corresponding to the IF3_mt is shown in orange.

(B) Molecular interpretation of the cryo-EM map in (A). The 12S rRNA is displayed in bright yellow, whereas a lighter shade of yellow is used to mark the 28S ribosomal proteins (PDB ID: 3JD5). Landmarks of the 28S subunit: h, head; b, body; pt, platform.

(C) Completed model of IF3_mt shown along with the corresponding cryo-EM density. Domains of IF3_mt are color coded as in (D).

(D) Overall domain organization of the human IF3_mt.

(E) Some of the segments of IF3_mt with better resolved densities.