Figure 3.

Interactions of the IF3_mt CTD with the 28S Subunit and its Anti-association Property

(A–D) (A and B) The CTD (orange) provides major anchoring points for the stable binding of IF3_mt by interacting with several 12S rRNA helices such as h24 (purple) and (C and D) h44 (cyan) and h45 (magenta). Thumbnails at the center represent overlaid positions of the ligands relative to the overall orientation to the 28S subunit (yellow).

(E) The CTD (orange) of IF3_mt in its present conformation would prevent the association of 39S subunit with the 28S subunit by sterically blocking the formation of the central inter-subunit bridge, B2a, between h44 of the 28S subunit with H69 (blue), as well as docking of the mito-specific P-site finger (PSF, protein mL40, pink) from the large subunit. Landmarks on the thumbnail: CP, central protuberance of the large subunit; rest of the labels are the same as in Figure 2.