FIG. 1.

A laser scanning photostimulation setup for optogenetic mapping of motor cortex in Thy1-ChR2 transgenic mice. (A) A mouse was anesthetized and the left motor cortex was photostimulated by a blue laser (470 nm) through a 5 × microscope objective, and the evoked motor response was detected by an electrode inserted in the gastrocnemius muscle of the right hindlimb. (B) Six stimulation regions were set in the left motor cortex, with two regions anterior and four regions posterior to bregma. There were 30 stimulation spots in each region and totally 180 stimulation spots on the left cortex. The white dot in (B) indicates the location of bregma, and the black dashed area indicates the area of optogenetic mapping. EMG, electromyogram.