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. 2019 Jan 7;12:87–101. doi: 10.1016/j.isci.2019.01.005

Figure 2.

Figure 2

Zfp238 Is Expressed in Adipose Tissues

(A) Western blot of Zfp238 in various tissues. Protein lysates from the indicated tissues were subjected to western blot with anti-Zfp238 and anti-tubulin antibodies.

(B) Western blot of Zfp238 using the stromal vascular and adipocytes fractions of fractionated WAT.

(C and D) Real-time PCR of Zfp238 from 3T3-L1 (C) and T37i cells (D) during differentiation. An asterisk indicates a statistically significant difference between day 0 and day 7 or 10 (*p < 0.05 by one-way ANOVA). Data represent the mean ± SEM from three independent experiments.

(E and F) Western blot of Zfp238 protein from 3T3-L1 (E) and T37i (F) cells during differentiation. Lysates from cells on the indicated day after induction of differentiation were subjected to western blot.