Zfp238 Is Expressed in Adipose Tissues
(A) Western blot of Zfp238 in various tissues. Protein lysates from the indicated tissues were subjected to western blot with anti-Zfp238 and anti-tubulin antibodies.
(B) Western blot of Zfp238 using the stromal vascular and adipocytes fractions of fractionated WAT.
(C and D) Real-time PCR of Zfp238 from 3T3-L1 (C) and T37i cells (D) during differentiation. An asterisk indicates a statistically significant difference between day 0 and day 7 or 10 (*p < 0.05 by one-way ANOVA). Data represent the mean ± SEM from three independent experiments.
(E and F) Western blot of Zfp238 protein from 3T3-L1 (E) and T37i (F) cells during differentiation. Lysates from cells on the indicated day after induction of differentiation were subjected to western blot.