Zfp238 Regulates Ucp1 Expression in 3T3-L1 Cells
(A) Time course of gene expression in differentiated 3T3-L1 cells stimulated with forskolin (FSK).
(B) Time course of gene expression in differentiated 3T3-L1 cells incubated at 31°C. Experiments were performed three times. Data at each time point represent the ratio of gene expression level at basal state and means ± SEM. *p < 0.05 by two-way ANOVA with Fisher's test.
(C and D) Effects of knockdown of Zfp238 in differentiated 3T3-L1 cells on Ucp1 and Ppargc1a expression induced by FSK (C) or 6-h incubation at 31°C (D). Experiments were performed three times. Data represent the ratio of gene expression level in 3T3-L1 cells infected with retroviruses encoding shRNA-SCR at basal state and means ± SEM. *p < 0.05 by one-way ANOVA.
(E and F) Effects of overexpression of Zfp238 on Ucp1 and Ppargc1a expression in differentiated 3T3-L1 cells. Experiments were done three times. Data represent the ratio of gene expression level in 3T3-L1 cells infected with retroviruses encoding FLAG empty vector (control) at basal state and means ± SEM. *p < 0.05 by one-way ANOVA.
(G) Normalization of data of chromatin immunoprecipitation assay. Experiments were performed three times. Data represent the percentage of density of input performed by real-time PCR and means ± SEM. *p < 0.05 by one-way ANOVA.